With this feature on, the program will mechanically retrieve the SNP info contained in template and avoid choosing primers within the SNP areas. Help The minimal variety of contiguous nucleotide base matches between the question sequence and the target sequence that is wanted for BLAST to detect the targets. Set a lower worth if you should discover goal sequences with extra mismatches to your primers. This permits our new graphic show that provides enhanced overview for your template and primers. Additionally this program has limit detecting targets which may be too different from the primers…it will detect targets that have up to 35{c339f69c8782c2b631f6cd2ea1dc7043792a120cbf972df9bb6917a4a6b64b63} mismatches to the primer sequences (i.e., a total of 7 mismatches for a 20-mer). Help This requires at least one primer to have the required number of mismatches to unintended targets.
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